Tissue culture urokinase
Urokinase
CAS: 9039-53-6
Molecular Formula: C21H25BrN2O3
Tissue culture urokinase - Names and Identifiers
Tissue culture urokinase - Physico-chemical Properties
| Molecular Formula | C21H25BrN2O3 |
| Water Solubility | Reconstitute in water at 100µg/ml |
| Solubility | Soluble in water. |
| Appearance | powder |
| Color | white |
| Storage Condition | 2-8°C |
| MDL | MFCD00132566 |
| Physical and Chemical Properties | Urokinase is a serine protease produced by human renal tubular epithelial cells. It is a white amorphous powder and easily soluble in water. Dilute solution is unstable and must be used fresh and should not be diluted with acidic solution. The freeze-dried state can be stable for several years. Isoelectric point PH8.4-8.7. Urokinase is a highly specific and selective proteolytic enzyme. The activity of this product on synthetic substrates is similar to that of trypsin and plasmin, and it also has the activity of esterase. No antigenicity, no antibodies are produced in the body. In vivo t1/2 is 14min ± 6min. |
| Use | Is the treatment of cerebral vascular thrombosis, myocardial infarction |
Tissue culture urokinase - Risk and Safety
| WGK Germany | 3 |
| RTECS | OB8900000 |
| FLUKA BRAND F CODES | 10-21 |
| TSCA | Yes |
Tissue culture urokinase - Reference
| Reference Show more | 1. Liu Yanmin, Shen Lu, Wang Kang et al. Isolation of Bacillus natto from traditional fermented soybean and its fermentation [J]. Food science 2020 41(2). 2. Su Min Yu Hong Liang Wang Shang et al. Preliminary Study on low-cost production of Nattokinase [J]. Food industry 2017(10):48-52. 3. Yin Mengwen, Zhou Qian, Lei Yan, et al. Screening and safety evaluation of fibrinolytic enzyme-producing lactic acid bacteria [J]. Food and machinery 2017 033(010):28-33. 4. Qian Zedong, Zhang Youhong, Lu Yubing, Zhu Na, Tian Li. Isolation, screening and identification of Bacillus natto [J]. Chemistry and bioengineering, 2018,35(08):41-44. 5. Zhang, Xing-can. A preliminary study on the stability of Nattokinase in fermented bean curd [J]. China condiment, 2015,40(02):72-76 80. 6. Li xiulang, Li Qianwen, Zhu Yu, Han Xiaoyun, Sun Qingshen, Song Yong. Extraction and characterization of Nattokinase from vegetable soybean fermented by Bacillus natto [J]. China Brewing, 2020,39(12):106-112. 7. Zhang Liqiang, ran Maofang, Wang Songtao, Liu Guangqian, Ma Zhuo, Ren Jianbo, Shen Caihong, Xu Tao. Isolation and identification of fibrinolytic enzyme-producing strain from Luzhou Laojiao Daqu [J]. Brewing Science & Technology, 2021(03):17-24. 8. Fan, J. J., et al. "Comparative assessment of in vitro causal and fibrotic activity of four aloe species and analysis of their pathological composition by LC-MS." South African Journal of Botany 119 (2018): 325-334.https://doi.org/10.1016/j.sajb.2018.1 9. [IF=7.727] Xing Pei et al."Local delivery of cardiac stem cells overexpressing HIF-1α promotes angiogenesis and muscular tissue repair in a hind limb ischemia model."J Control Release. 2020 Jun;322:610 10. [IF=2.162] Yajie Duan et al."Production purification and characterization. "Preparative Biochemistry & Biotechnology. 2022 Jan 09 Note: For some products, we can only provide some information. We do not guarantee the authority of the information provided. use: This product is for scientific research only, not for other purposes. storage conditions: -20 ℃ sensitivity: sensitive to moisture solubility: soluble in water stability: dry powder is stable at 4 ℃, the aqueous solution was stable for 3 days at 4 °c. vitality Definition: Unit Definition One unit will activate that amount of minimal which will produce a ΔA275 of 1.0 per ml per minute at pH 7.5 at 37°C, when measuring perchloric acid soluble products from α-casein (1cm light path). |
Tissue culture urokinase - Nature
Open Data Verified Data
Urokinase is a serine protease produced by human renal tubular epithelial cells and is a mixture of high molecular weight urokinase (HUK,54000) and low molecular weight urokinase (LUK,33000). It is a specific and highly selective proteolytic enzyme. Plasminogen is the only natural protein substrate, this product acts on the plasminogen arginine-valine bond to plasminogen into active plasmin. This product on the synthesis of substrate activity and trypsin and plasmin approximation, but also has the activity of esterase. The isoelectric point pl was 8.4-8.7. No antigenicity, do not make the body produce antibodies. In vivo tl/2 was (14±6) min. Freeze-dried state can be stable for several years, dilute solution is unstable, must be fresh. This product is white non-crystalline powder, soluble in water.
Last Update：2024-01-02 23:10:35
Tissue culture urokinase - Preparation Method
Open Data Verified Data
- adsorption method [diatomite: 724 resin (H + type);D-160 resin] Fresh male urine (pH6.5 or less, bacterial count 1000/mL or less) is cooled to below 10 ℃, the pH was adjusted to 8.5 with 3mol/L sodium hydroxide, and th was allowed to stand. The supernatant was siphled off and re-neutralized to pH 5-5.5 with 3mol/L hydrochloric acid. Each liter of acidified urine was then added to the diatomaceous earth lOg, and the adsorption was stirred below 5 °c. After the adsorbate was washed with cold water at 5 °c, the column was loaded with 0. 02% ammonia water was washed until the effluent became clear, and then ammonia water containing sodium chloride (1 mol/L) (0. 02%) the elution of urokinase, in the elution of turbid liquid began to collect, the amount of collection of about 15L per ton of urine elution. The eluent was adjusted to pH 8 with saturated sodium dihydrogen phosphate and to conductance 22M. Q -1 with sodium chloride. The effluent was then collected through a QAE-Sephadex column, which was washed again with phosphate buffer and combined with the effluent. The pH value of the effluent was adjusted to 4.2 with acetic acid, and the conductance was adjusted to 16-17m with distilled water. Then through the CMC column, with pH 4.2 acetic acid sodium acetate buffer solution to wash the column, and then containing sodium chloride (0. 1 mol/L) of ammonia (0. 1%) elution of urokinase. The eluate was dialyzed against water at 4 ℃ for 24h, and the dialysate was centrifuged to precipitate and freeze-dried to obtain urokinase.
- tissue culture method (US).
Last Update：2022-01-01 11:10:56
Tissue culture urokinase - Standard
Authoritative Data Verified Data
an enzyme that activates plasminogen extracted from fresh human urine. It is a mixture of high molecular weight urokinase (Mw54000) and low molecular weight urokinase (Mw33000), the content of high molecular weight urokinase should not be less than 90%, and the activity of urokinase should not be less than 120,000 units per 1 mg protein.
Last Update：2024-01-02 23:10:35
Tissue culture urokinase - Legal requirements
Authoritative Data Verified Data
This product should be extracted from the urine of healthy people, and the production process should comply with the requirements of the current version of "good manufacturing practice. In the production process, this product needs to be heated at 60°C for 10 hours to make Virus inactivation.
Last Update：2022-01-01 11:59:40
Tissue culture urokinase - Introduction
Urokinase is a protease that cleaves plasminogen (plasminogen) to form plasmin (plasmin). It binds to cell surface receptors (uPAR), regulates cell adhesion tissue remodeling and migration, and also activates intracellular signal transduction pathways. Urokinase is composed of three domains: the amino-terminal domain homologous to epidermal growth factor, which is a protease domain bound to the carboxyl terminal through the central kringle domain.
Last Update：2022-10-16 17:30:00
Tissue culture urokinase - Use
Open Data Verified Data
- proteolytic enzyme. Highly efficient thrombolytic agent, can promote the dissolution of thrombus, there are expansion of local vascular role. Clinical for acute myocardial infarction, unstable angina; Treatment of cerebrovascular disease, arterial embolism disease, thrombotic thrombocytopenic purpura, systemic lupus erythematosus; Can also be used for the treatment of cancer.
- usage and dose of intravenous drip, 1~20,000 units each time, added into glucose solution, 1~2 times a day, for 5~7 days, then intramuscular injection of 5000 units daily to maintain; intravenous injection, starting 2-3 days 15,000-20,000 units, 2 times, then daily 10,000-20,000 units, 7-10 days.
- before injection, add Sterile Water for Injection to dissolve. Ophthalmic local injection, dissolved in appropriate sterile water for injection, make isotonic solution. 150-500 units (u) once a day. Acute myocardial infarction, a 500,000~1.5 million units, dissolved in sodium chloride injection or 5% glucose injection 50 ~ lOOmL intravenous infusion or 200,000~1 million units dissolved in sodium chloride injection or 5% glucose injection 20 ~ 60mL, intracoronary perfusion.
Last Update：2022-01-01 11:10:57
Tissue culture urokinase - Trait
Authoritative Data Verified Data
This product is white or off-white powder.
Last Update：2022-01-01 11:59:40
Tissue culture urokinase - Safety
Open Data Verified Data
- rats and mice were not dead by intravenous injection of 1 × l06 u/kg, so LD50 >1 × l06 u/kg. This product is easy to cause bleeding. If the bleeding tendency is found, the drug should be discontinued immediately, and the antifibrinolytic drug should be used to combat the drug. Not with other anticoagulants or platelet inhibitors. This product should be used immediately after dissolution, and should not be diluted with acidic infusion. Bleeding diathesis, liver and kidney insufficiency, ulcer disease, malignant hypertension, cerebral hemorrhage, hemophilia and subacute endocarditis, threatened abortion, pregnant women and after delivery and maternal surgery disabled.
- monitoring of blood coagulation should be performed during the administration of injection. Patients with severe hypertension, severe liver disease and bleeding tendency should be used with caution. This product should not be diluted with acidic injection, so as not to reduce the efficacy.
- light shielding, closed, and stored at 10 °c or less.
Last Update：2022-01-01 11:10:58
Tissue culture urokinase - Differential diagnosis
Authoritative Data Verified Data
The Test Solution of the potency determination item is diluted with barbiturate-sodium chloride buffer (pH 7.8) to make a solution containing 20 units per 1 ml, 0.3 of bovine fibrinogen solution was added, followed by 0.2ml of human bovine plasminogen solution and 0.2 of bovine thrombin solution, immediately place 37°C ± 0.5°C in a constant temperature water bath, and immediately time. The clot should clot within 30-45 seconds and redissolve within 15 minutes. With 0.9% sodium chloride solution as a blank, the same method operation, the clot is insoluble in 2 hours (the preparation of the above reagent with potency determination).
Last Update：2022-01-01 11:59:41
Tissue culture urokinase - Exam
Authoritative Data Verified Data
clarity and color of solution
This product is dissolved and diluted with 0.9% sodium chloride solution to make a solution containing 3000 units per 1 ml, which is checked according to law (General rule 0901 first law and general rule 0902 first law), should be clear and colorless.
loss on drying
take this product, with phosphorus pentoxide as desiccant, at 60°C under reduced pressure drying to constant weight, weight loss should not be greater than 5.0% (General 0831).
molecular component ratio
take this product, add water to dissolve and dilute the solution containing 2mg per lml, add equal volume of buffer [take concentrated gel buffer (F solution) 2-5ml, 20% sodium dodecyl sulfate solution (2.5ml), 0.1% bromophenol blue solution (87% ml) and 3.5 glycerol solution (ml), add water to 10 ml], place in a water bath for 3 minutes, and let cool, add to sample well, according to the electrophoresis method (General 0541 fifth method Coomassie Brilliant Blue staining) determination.
hepatitis B surface antigen
take this product, add 0.9% sodium chloride solution to dissolve and dilute the solution containing lOmg in each lml, according to the kit instructions, it should be negative.
abnormal toxicity
take this product, add sodium chloride injection to dissolve and dilute the solution containing 5000 units per lml, check according to law (General 1141), should comply with the provisions. Bacterial endotoxin from this product, according to the inspection (General 1143), each 10,000 units of urokinase containing endotoxin should be less than 1.OEU.
thrombin-like active substance
- preparation of plasma fresh rabbit blood was taken, 3.8% sodium citrate solution was added (1 ml of 3.8% sodium citrate solution was added per 9ml rabbit blood), mixed well, and at 2-8°C, centrifuge at 5000 rpm for 20 minutes. The solution was frozen at one 20°C for storage, and thawed at 25°C before use.
- determination of this product, plus barbiturate buffer (pH 7.4) dissolved and diluted to make each 1 ml containing 5000 units, 2500 units, 1250 units, 625 units and 312 units of the test solution. If the test product contains ethylenediamine tetraacetate or phosphate, it must be first removed by dialysis with barbiturate buffer (pH 7.4) at 2°C, and then prepared as a solution of the above concentration.
- seven small tubes (12mm x 75mm) were taken, and barbiturate buffer (pH 7.4) was added to tubes 1 and 7, respectively. 1ml as blank control, the remaining 5 tubes were respectively added with 0.1ml of the test solution diluted by the above ratio, and then added with 6-aminocaproic acid solution in turn [1.97G of 6-aminocaproic acid], dissolve with barbiturate buffer (pH 7.4) and dilute to 50ml] and 0.1ml each of plasma, gently shake, and stand in a water bath at 25°C for 3 minutes, add 0.1 of calcium chloride solution (take 1.84g of calcium chloride, add water to dissolve and dilute to 500ml) which has been pre-warmed to 25°C, mix well, place in water bath, immediately time. Pay attention to the observation of Plasma Coagulation. The end point was judged as gently inclined test tube in horizontal shape, the solution was inclined but not flowing, average (the difference between the maximum value and the minimum value in 3 determinations shall not exceed 10% of the average). The logarithm of the concentration of the test solution is taken as the ordinate, and the time of shortening the calcium complex (the coagulation time of the blank tube minus the coagulation time of the test tube) is plotted as the abscissa. Each point of the test sample with different dilutions should be connected in a straight line, and the intersection point of the extension line and the ordinate axis is the concentration of the test sample, that is, the enzyme activity of the test sample when the thrombin activity is zero, expressed in units per 1ml of test solution, should not be less than 150 units per 1 ml.
Last Update：2022-01-01 11:59:42
Tissue culture urokinase - Titer determination
Authoritative Data Verified Data
enzyme activity
- reagent bovine fibrinogen solution bovine fibrinogen was taken, dissolved and diluted with a barbiturate-gasified sodium buffer (pH 7.8) to prepare a solution containing 6.67mg of coagulable protein per 1 ml. BOVINE THROMBIN solution bovine thrombin was taken, dissolved and diluted with barbiturate-sodium chloride buffer (pH 7.8) to make a solution containing 6.0 units per 1 ml. Bovine plasminogen solution a solution containing 1 to 9.0 casein units per 1 mL (if the solution is turbid) is prepared by dissolving and diluting bovine plasminogen in Tris buffer (pH 1.4), centrifugation, take the supernatant for use). The mixed solution was mixed with equal volume of bovine thrombin solution and bovine plasminogen solution before use.
- preparation of standard solution a urokinase standard was taken, dissolved in a barbiturate-gasified sodium buffer (pH 7.8), and quantitatively diluted to prepare a solution containing 60 units per lm l.
- preparation of Test Solution take the right amount of this product, precision weighing, plus barbiturate-gasification sodium buffer solution (pH 7.8) dissolved, and quantitative dilution of the solution prepared with the same concentration of standard solution, shake.
- test tube 4, each with 0.3 of bovine fibrinogen solution, was placed in 37°C ± 0.5°C water bath, with 7.8 of barbiturate-sodium chloride buffer (pH 0.9), 0.8ml, 0.7ml, 0.6ml, followed by standard solution 0.lml, 0.2ml, 0.3ml, 0.4ml, and then mixed solution 0.4ml, immediately shake, respectively. The reaction system should be coagulated within 30 to 40 seconds, and when the small bubbles in the clot rise to half of the volume of the reaction system, the end point of the reaction is immediately timed. Each concentration is measured 3 times, and the average value is calculated (the difference between the maximum value and the minimum value in the 3 determinations shall not exceed 10% of the average value). Linear regression was performed using the logarithm of the urokinase concentration as the abscissa and the logarithm of the reaction endpoint time as the ordinate. The test article is measured according to the above method, the concentration of the test article solution is obtained by linear regression equation, and the titer (unit) per 1 mg of the test article is calculated.
Protein
The content of this product is about 10 mg, precision weighing, according to the protein content determination method (General 0731 first method) determination, that is. Specific activity the number of units of urokinase activity per 1 mg of protein.
Last Update：2022-01-01 11:59:42
Tissue culture urokinase - Category
Authoritative Data Verified Data
thrombolytic drugs.
Last Update：2022-01-01 11:59:43
Tissue culture urokinase - Storage
Authoritative Data Verified Data
light shielding, sealing, and storage at 10°C or lower.
Last Update：2022-01-01 11:59:43
Tissue culture urokinase - Urokinase for Injection
Authoritative Data Verified Data
This product is a sterile lyophilized product of urokinase plus an appropriate amount of stabilizer and excipient. The urokinase activity unit of this product should be 85.0% to 120.0% of the labeled amount.
trait
This product is a white or off-white lyophilized cake or powder.
identification
According to the identification test under the item of urokinase, the same results were shown.
examination
- pH value: take this product, add 2ml of water to dissolve, mix well, and measure according to law (General rule 0631). The pH value should be 6.0~7.5.
- clarity and color of solution take this product, add 0.9% sodium chloride solution to dissolve and dilute to make a solution containing 3000 units per 1 ml, inspection according to law (General rule 0901 first law and general rule 0902 first law) shall be clear and colorless.
- weight loss on drying this product, with phosphorus pentoxide as desiccant, drying under reduced pressure at 60°C for 4 hours, the weight loss shall not be greater than 5.0% (General 0831).
- sterile take this product, after dissolving with appropriate solvent, the membrane filtration method, according to the law inspection (General 1101), should comply with the provisions.
- bacterial endotoxin should be checked according to the method under urokinase, and it should be in accordance with the regulations.
- others should comply with the relevant provisions under injection (General 0102).
titer determination
take 5 pieces of this product, add appropriate amount of barbiturate-gasified sodium buffer (pH 7.8) to dissolve, and transfer the whole amount to the same 100ml measuring flask, dilute to the scale with the above buffer, shake well. Precision take appropriate amount, quantitative dilution with the above buffer solution of appropriate concentration (should be in the standard curve concentration range under the urokinase method for the determination of enzyme activity, that is.
category
same with urokinase.
specification
(1)5000 units (2) 10,000 units (3) 50,000 units (4) 1=0 units (5) 200,000 units (6) 250,000 units (7) 5=0 units
(8)1 million units (9) 1.5 million units
storage
It was sealed and stored at 10°C or lower.
Last Update：2022-01-01 11:59:44
Tissue culture urokinase - Reference Information
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| drug interaction | the interaction between urokinase and other drugs has not been reported. In view of this product is a thrombolytic drug, therefore, the impact of platelet function of drugs, such as aspirin, indomethacin, Bao Tai Song and other should not be combined. Heparin and oral anticoagulants should not be used with large doses of this product at the same time, so as not to increase the risk of bleeding. |
| adverse reaction | the toxicity of urokinase was very low, and the median lethal dose of intravenous injection in mice was more than 1 million international units/kg body weight. No obvious antigenicity, teratogenicity, carcinogenicity and mutagenicity. Clinical application of rare reports of allergic reactions. However, given that this product increases Plasmin Activity and reduces circulating unbound plasminogen and fibrin-bound plasminogen, there may be a serious risk of bleeding. The adverse reaction of urokinase was also less than that of streptokinase. There are a variety of bleeding, occasional rash, urticaria and other allergic reactions, there are Fever, Head Pain and Nausea, Vomit, loss of appetite and other gastrointestinal reactions. Few antigen reactions. |
| pharmacological action and mechanism of action | urokinase directly acts on the endogenous fibrinolysis system and catalyzes the cleavage of plasminogen to plasmin, the latter can not only degrade fibrin clot, but also degrade fibrinogen, coagulation factor V and coagulation factor VIII in the blood circulation, so as to play a thrombolytic effect. This product on the formation of new thrombus rapid onset, good effect. This product can also improve vascular ADP enzyme activity, inhibit ADP-induced platelet aggregation and prevent thrombosis. After intravenous infusion of this product, the activity of plasmin in the patient's body was significantly improved; After stopping the drug for a few hours, the activity of plasmin was restored to the original level. However, the decrease in plasma fibrin or fibrinogen levels, as well as the increase in their degradation products, can last from 12 to 24 hours. This product showed a clear correlation between thrombolytic effect and drug dose, administration time. |
| Use | Urokinase is a proteolytic enzyme. It can be used as a highly efficient thrombolytic agent, and has the effect of expanding local blood vessels. Monitoring bed is mainly used for acute myocardial infarction, unstable angina, treatment of cerebrovascular disease, arterial embolism disease, thrombotic thrombocytopenic purpura, systemic lupus erythematosus; Can also be used for the treatment of cancer. But after the use of this product is easy to cause bleeding, such as the discovery should be immediately discontinued. Not with other anticoagulants or platelet inhibitors. Monitoring and observation of blood coagulation should be made during administration. Bleeding quality, malignant hypertension, ulcer disease, liver and kidney dysfunction, hemophilia and subacute endocarditis, threatened abortion, pregnant women and after delivery and maternal and surgical operation disabled. LD 50>1 x 106U/kg. Protected from light, sealed, stored below 100. it is useful as an antithrombotic agent for cerebral embolism, deep venous thrombosis, peripheral arteriovenous thrombosis, pulmonary embolism, myocardial infarction, and reticular arteriovenous thrombosis. Urokinase is a specific drug for the treatment of cerebrovascular thrombosis and myocardial infarction Urokinase is a protease that cleaves plasmin to form plasmin. It binds to cell surface receptors (upars), regulates tissue remodeling and migration, and also activates intracellular signal transduction pathways. Urokinase is composed of three domains: an amino-terminal domain homologous to epidermal growth factor (epidermal growth factor), a protease domain that binds to the carboxy terminus through a central kringle domain. |
| production method | Urokinase is produced by kidney cells and is present in urine but not in blood. Per 100 milliliters of human urine, contains 1-2 micrograms of urokinase, roughly equivalent to 200-400 international units. Can be extracted from human urine. male urine was collected by adsorption, precipitation and acidification in a special plastic barrel. The conductance of urine is 20-30m · ↑-1, Ph is below 6.5, and the number of bacteria is below 1000/ml, 0.8% phenol was added for preservation in summer. Urine must be processed within 8H. The freshly treated urine was cooled to below 1010C, adjusted to Ph 8.5 with sodium hydroxide (3nol/L), allowed to stand for 1H, siphon the supernatant, with hydrochloric acid (3nol/L). Neutralize OH-1 to pH 5-5.5. Acidified urine. Fresh male urine NaOH, pH 8 → precipitated supernatant urine HCl, pH 5 → acidified urine adsorption, elution to obtain acidified urine, adding 10g(1%) of diatomite per liter of urine, the adsorption was carried out for 1H under constant stirring below 50 °c. The adsorbate was washed with about 50 cold water, packed with column (column ratio 1:1), washed with ammonia water (0.02%) until the effluent solution became clear, and then contained sodium chloride (1mol/L). The urokinase was eluted with ammonia (0.02%) until collection was started when the eluate was changed to mix, and the collected amount was about 15L of eluate (100U/ml,3000U/mg) per ton of urine. Acidizing urine diatomite → adsorbing and adsorbing cold water 50 ^ → washing diatomite column NH3 ^ · H2O → washing eluent to remove Pyrogen and pigment eluent adjust pH 8 with saturated sodium dihydrogen phosphate, the conductance was adjusted to a value corresponding to 22mm · I-1 with sodium chloride, and the effluent was collected through a QAE-Sephadex chromatography column (the column was previously equilibrated with Ph 8R phosphate buffer). The column was continuously washed with phosphate buffer (not more than 3 times the column volume retained) and combined with the effluent. Eluent QAE-Sephadex column → depyrogen and pigment effluent CMC concentration the effluent was adjusted to pH 4.2 with acetic acid (1mol/L) and conductance equivalent to 16-17 m↑ -1 with distilled water, pass through CMC chromatography column (this column is pretreated with 0.1mol/L, pH 4.2 acetic acid to buffer the solution, conductance equivalent to 17 mδ-1, equilibrium about 12h) continue to wash the chromatographic column with acetic acid-sodium acetate buffer solution with pH of not less than 10 times the volume of the column. After the elution is finished, add sodium chloride (0.1mol/L) and ammonia water (0.1%), the urokinase was eluted at pH 11.8~3000, and the urokinase eluate was discharged into filamentous form, and the eluate was partially collected (15000 ~ 40000U/ml, ~ 20000U/mg). Effluent CMC column → concentration} CMC column NH3 · H2,Ph11.5 → washing eluent dialysis, freeze-drying the eluent was dialyzed against 40 pairs of water for 24h to remove salt, water was changed for 3-4 times, and the dialysate was centrifuged to precipitate, urokinase was obtained by freeze-drying. The eluent water was 40;24H ^ → the finished product of dialysis and freeze-drying was sterilized and sub-packed. After The urokinase was qualified, it was diluted and sterilized, and the excipient was added, sub-packed and freeze-dried to obtain the urokinase preparation. Urokinase excipient → sterilized, lyophilized urokinase preparation |
Last Update：2024-04-09 22:09:11
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Product Name: Urokinase Visit Supplier Webpage Request for quotationCAS: 9039-53-6
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Product Name: Urokinase Request for quotationCAS: 9039-53-6
Tel: +86-17551318830
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Product Name: Urokinase Request for quotation
CAS: 9039-53-6
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